Low cost double optical tweezers to manipulate microorganisms and micro-crystals
J. Vargas-Valencia, J. P. Galaup
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Base Information
Volume
V49 - N4 / 2016 Ordinario
Reference
188-194
DOI
http://dx.doi.org/10.7149/OPA.49.4.48551
Language
English
Keywords
Optical tweezers, micromanipulation, micro crystals, micro machines
Abstract
An inexpensive double optical tweezers has been used for trapping and manipulating microorganisms and micro-sized crystals with regular and irregular shapes. The intensity and spatial location of the traps were controlled through mirror displacements devices. A stable infrared laser beam with tunable power was used. The particles were manipulated and observed in an inverted microscope adjusted to locate the infrared trapping laser beam into the holder. A bacilliform bacterium with an approximate size of 12.0?m was captured by its two elongated ends, exerting control of their displacements and rotations. Also, regular micro-crystals measuring approximately 10.0?mx4.0?mx0.5?m were captured, achieving nanometric control of their displacements and rotations in two dimensions, parallel to one of their faces.
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