Applications in light-induced spectroscopy with violet LED lamp: autofluorescence
M.E. Etcheverry, M.A Pasquale, M. Garavaglia
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Base Information
Volume
V56 - N3 / 2023 Ordinario
Reference
51120
DOI
http://dx.doi.org/10.7149/OPA.56.3.51120
Language
English
Keywords
Non-melanoma skin cancer, one-point fluorescence detection, violet LED lamp
Abstract
The early detection of a neoplastic disease, as well as the development of more efficient treatments, are crucial for improving the survival rate. A variety of new and emerging diagnostic strategies based on spectroscopic techniques such as non-invasive one-point fluorescence (PF) detection, are available to improve the screening procedure [1,2]. PF technique provides useful information for monitoring the evolution of the abundance and distribution of endogenous fluorophores associated with the neoplastic disease in low-pigmented superficial neoplasia [3,4]. The main objective of the present study was to develop a violet LED light source suitable for medical application devoted to the diagnostic and treatment of non-melanoma skin cancers. For this propose, we constructed a 12 W violet LED lamp (with maximum emission peak at 405 nm) made up of four mobile 3 W LED, each one coupled to a heat sink and a lens and mounted on a platform with adjustable screws. The power supply allows the modification of the intensity of illumination. The LED lamp was characterized employing a spectrometer coupled to an optical fiber. The irradiance for different distances between the lamp and the detector was assessed. Furthermore, the radiant power and the radiant intensity were evaluated. Data were compared with a simulated LED lamp by using the Zemax optic software for the realization of luminaries with the desired characteristics and modes of operation to deliver the energy density at the interest point, as required for medical applications. The developed lamp in combination with a portable spectrometer was employed under medical supervision to detect differences in the emission spectrum of skin suspicious regions and healthy ones located at the head of a patient with non-melanoma skin cancer. The light of the LED lamp was concentrated in the examined region by means of the proper focusing of the four individual LEDs, rendering a high intensity homogeneous spot. Thus, endogenous chromophores at the skin were excited, and the emission intensity appeared to be enough to detect an enhanced peaked structure around 600 nm for some suspicious regions before treatment, and that were absence in healthy regions. These differences can be related to the augmented protoporphyrin IX content in neoplastic regions. Results presented in this work indicate the usefulness of the developed and characterized LED lamp as an easy-to-use device for the non-invasive detection of skin neoplastic pathologies, before and after treatment, with the aim of better define the malignant regions as well as predict the outcome of a certain treatment.
References
Q. Liu, “Role of Optical Spectroscopy Using Endogenous Contrasts in Clinical Cancer Diagnosis,” World J Clin Oncol 10, 50-63 (2011).
D. van Straten, V. Mashayekhi, H.S. de Bruijn, S. Oliveira, D.J. Robinson, “Oncologic Photodynamic Therapy: Basic Principles, Current Clinical Status and Future Directions,” Cancers (Basel) 9(19), 2-54 (2017).
E. Borisova, L. Avramov, P. Pavlova, E. Pavlova, P. Troyanova, “Qualitative Optical Evaluation of Malignancies Related to Cutaneous Phototype,” Proc. SPIE –Dynamics and Fluctuations in Biomedical Photonics VII, 75630X (2010).
M. Panjepour, C. Julius, M. Phan, T. Vo-Dinh, S. Overholt, “Laser-induced Fluorescence Spectroscopy for in vivo Diagnosis of Non-melanoma Skin Cancers,” Lasers Surg Med 31, 367-373 (2002).
J. Bigio, J.R. Mourant, “Ultraviolet and Visible Spectroscopies for Tissue Diagnostics: Fluorescence Spectroscopy and Elastic-scattering Spectroscopy,” Phys Med Biol 42, 803-814 (1997).
R. Na, I. Stender, H. Wulf, “Can Autofluorescence Demarcate Basal Cell Carcinoma from Normal Skin? A Comparison with Protoporphyrin IX Fluorescence,” Acta Derm-Venerol 81, 246-249 (2001).
A. Pena, M. Strupler, T. Boulesteix, G. Godeau, M.-C. Schanne-Klein, “Spectroscopic Analysis of Keratin Endogenous Signal for Skin Multiphoton Microscopy,” Opt Express 13, 6268-6274 (2005).
N. Ramanujam, “Fluorescence Spectroscopy of Neoplastic and Non-neoplastic Tissues,” Neoplasia 2, 89-117 (2000).
A. Leunig, K. Rick, H. Stepp, R. Gutmann, G. Alwin, R. Baumgartner, J. Feyh, “Fluorescence imaging and spectroscopy of 5-aminolevulinic acid induced Protoporphyrin IX for the dectection of neoplastic lesions in the oral cavity,” Am J Surg 172, 674-677 (1996).
H. Kostron, A. Zimmermann, A. Obwegeser, “mTHPC-mediated photodynamic detection for fluorescence-guided resection of brain tumors," Proc SPIE 3262, Surgical-Assist Systems (1998).
J.S. Tyrrell, S.M. Campbell, A.Curnow A, “The relationship between protoporphyrin IX photobleaching during real-time dermatological methyl-aminolevulinate photodynamic therapy (MAL-PDT) and subsequent clinical outcome,” Lasers Surg Med 42,613-619 (2010).
G.A. Wagnières, W.M. Star, B.C. Wilson, “In vivo fluorescence spectroscopy and imaging for oncological applications,” Photochem Photobiol 68, 603-632(1998).
E. Borisova, L. Avramov, “Laser System for Optical Biopsy and in vivo Study of the Human Skin,” Proc SPIE 4397, 405-409 (2000).
L. Bachmann, D. Zezell, A. da Costa Ribeiro, L. Gomes, A. Ito, “Fluorescence Spectroscopy of Biological Tissues,” Appl Spectr Rev 41, 575-590 (2006).
R.E. Fischer, B. Tadic-Galeb, P.R. Yoder, Optical System Design, 2nd ed., McGraw-Hill, New York, (2008).
W.J. Smith, Modern Optical Engineering, 4th ed., McGraw-Hill, United State, (2007).
J.M. Geary, Introduction to Lens Design: With Practical Zemax Examples. Willmann-Bel, (2002).
I. Moreno, C-C. Sun, “Modeling the radiation pattern of LEDs,” Opt Express 16,1808-1819 (2008).
K.R. Rollakanti, S.C. Kanick, S.C. Davis, B.W. Pogue, E.V. Maytin, “Techniques for fluorescence detection of protoporphyrin IX in skin cancers associated with photodynamic therapy,” Photonics Lasers Med 2, 287-303 (2013)
N. Rajaram, D. Kovacic; M.F. Migden, J.S. Reichenberg, T.H. Nguyen, J.W. Tunnell, “In vivo determination of optical properties and fluorophore characteristics of non-melanoma skin cancer,” Proc SPIE 716102, 1-9 (2009).
I. Bliznakova, E. Borisova, L. Avramov, “Laser and Light-Induced Autofluorescence Spectroscopy of Human Skin in Dependence on Excitation Wavelengths,” Acta Phys Pol A 112, 1131-1136 (2007).
I. Wang, K. Svanberg, S. Andersson-Engels, R. Berg, S. Svanberg, “Photodynamic therapy of non-melanoma skin malignancies with topical δ-amino levulinic acid: diagnostic measurements,” Proc SPIE 2371, 243-252 (1995).
K. Malzahn, T. Dreyer, H. Glanz, C. Arens, “Autofluorescence endoscopy in the diagnosis of early laryngeal cancer and its precursor lesions,” Laryngoscope 112(3), 488-493 (2002).
M. Zargi, I. Fajdiga, L. Smid, “Autofluorescence imaging in the diagnosis of laryngeal cancer,” Eur Arch Otorhinolaryngol 257, 17-23 (2000).
C. Arens, D. Reussner, H. Neubacher, J. Woenckhaus, H. Glanz, “Spectrometric measurement in laryngeal cancer,” Eur Arch Otorhinolaryngol 263(11), 1001-1007(2006).
O. Aboumarzouk, R. Valentine, R. Buist, S. Ahmad, G. Nabi, S. Eljamel, H. Moseley, S.G. Kata, “Laser-induced autofluorescence spectroscopy: Can it be of importance in detection of bladder lesions?,” Photodiagnosis Photodyn Ther 12, 76-83 (2015).