Polarimetric multiphoton microscopy for the analysis of ocular structures

F. J. ávila, O. del Barco, J. M. Bueno

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Base Information

Volume

V52 - N1 / 2019 Ordinario

Reference

51013:1-9

DOI

http://doi.org/10.7149/OPA.52.1.51013

Language

Spanish

Keywords

Non-linear microscopy, second harmonic generation, polarization, collagen

Abstract

This review article summarizes the main contributions of the PhD Thesis titled "Polarization and multiphoton microsocopy for the analysis of ocular structures" carried out at the Laboratorio de óptica of the University of Murcia. This work presents mathematical methods developed to both analyze and classify the external or micrometric scale of type I collagen-based ocular tissues. On the one hand, a theoretical model has been developed to explore the polarization dependence of second harmonic generation signal. This allowed obtaining an analytical expression to calculate the internal or molecular collagen organization. On the other hand, an experimental relationship between external and internal collagen organization has been stablished. Finally, the Stokes-Mueller formalism has been applied to compute the polarization properties of type-I fibrillar collagen and to improve the visualization of local structures. These methods have been proposed as a differential clinical tool for the diagnosis of corneal keratoconus.

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