Terapia fotodinámica con fotosensibilizadores no porfirinos en células de mama cancerígenas y no cancerígenas
Juan Carlos Atenco-Cuautle, Nayeli Perez-Perez, Maria Guadalupe Delgado-Lopez, Julian Ramirez-Ramirez, Julio Cesar Ramirez-San-Juan, Ruben Ramos-Garcia, Teresita Spezzia-Mazzocco
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Información básica
Volumen
V57 - N1 / 2024 Especial: RIAO OPTILAS 2023
Referencia
51161
DOI
http://dx.doi.org/10.7149/OPA.57.1.51161
Idioma
English
Etiquetas
Cáncer de mama, terapia fotodinámica, fotosensibilizadores no porfirínicos
Resumen
El cáncer de mama es una de las principales patologías que causan altas tasas de morbilidad y mortalidad en las mujeres a nivel mundial. Las terapias convencionales tienen efectos secundarios indeseables y en algunos casos, son ineficaces; por ello, la búsqueda de terapias alternativas, como la terapia fotodinámica (TFD), es fundamental. En este trabajo se investigó el efecto de la TFD sobre dos líneas celulares de cáncer de mama y una línea celular de mama no cancerígena, utilizando fotosensibilizadores (FS) no porfirínicos, como el azul de metileno (AM) y el rosa de bengala (RB). La eficacia de cada tratamiento se evaluó midiendo la viabilidad celular con el ensayo MTT y mediante observaciones morfológicas. Antes de evaluar los efectos de la TFD, se midió la absorción intracelular de cada FS en las líneas celulares estudiadas. Los resultados obtenidos mostraron que la línea celular MDA-MB-231, presentó la mayor sensibilidad a la PDT para ambos tratamientos de TFD. La línea celular T47D presentó casi un 50% de inhibición en ambos casos. Sorprendentemente, la línea celular MCF10A, no cancerígena, mostró una respuesta selectiva a la TFD. En particular, no se observó una inhibición importante empleando el tratamiento con MB-PDT. Sin embargo, si se observó inhibición con el tratamiento de RB-PDT. Los cambios morfológicos respaldaron el efecto citopático inducido por la TFD, donde fue evidente que las células MCF10A no cancerosas mostraron cambios notables solo con la dosis más alta de RB-PDT. En conclusión, este estudio presenta por primera vez el efecto de la TFD utilizando RB como fotosensibilizador para la inhibición de las células mamarias MDA-MB-231, T47D y MCF10A, y usando MB-PDT para la inhibición de las células T47D. Por último, no se observó una relación directa entre el efecto de la TFD y la concentración intracelular del FS
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